Detection of IL-15 in stimulated cord (CB) and adult (APB) monocytes. Immunoblot analysis of LPS (12 hours) stimulated CB and APB monocytes using anti–huIL-15 goat antibody. Equal amounts of protein (20 μg) were loaded per lane. Before loading, quantitation of total protein from each sample was performed in duplicate, twice using BCA protein assay. Lane 1, rhIL-15; lane 2, adhered monocytes; lanes 3 and 4, LPS-stimulated adult monocytes; lanes 5 and 6, LPS-stimulated cord monocytes. The lower panel bar graph represents comparative IL-15 protein production from three different LPS-stimulated (12 hours) CB and APB monocytes. The amount of IL-15 protein was expressed as a percentage, setting the amount of APB level equal to 100% (APB v CB, 100% v 30% ± 2.5%, P < .05, n = 3).