ICSBP-transcript numbers in mononuclear cells of a CML patient during in vitro stimulation with IFN-α and IFN-γ. An initial control was obtained (lane 1); samples were obtained after 6 hours (lanes 2, 5, and 8), after 24 hours (lanes 3, 6, and 9) and after 48 hours (lanes 4, 7, and 10). No increase ofICSBP-transcript numbers was seen without IFN (lanes 2 through 4) or with IFN-α treatment (1,000 U/mL; lanes 5 through 7). Only during IFN-γ treatment (1,000 U/mL) ICSBP-transcript numbers increased significantly (lanes 8 through 10). The mean of three experiments is displayed (±SEM).