Fig. 6.
rhIL-11 induced tyrosine phosphorylation of gp130 and STAT3 proteins in BM MKs. Isolated guinea pig BM MKs were incubated with or without rhIL-11 after a preincubation with 32P-orthophosphate. Cells were lysed and supernatants were incubated with anti-gp130 antibody (A) and anti-STAT3 antibody (B). Immunoprecipitates were separated by SDS-PAGE and autoradiography of the dried gels was performed.
