Tyrosine phosphorylations of platelet proteins before and after the stimulation with rhTPO. (A) The platelets from three ET patients (P4, P10, and P16) and one normal control (C1) were stimulated with rhTPO (200 ng/mL) for 10 minutes. The lysates were separated by SDS-PAGE and probed with anti-phosphotyrosine MoAb. (B) The platelets from an ET patient (P16) and a normal control (C1) were stimulated with rhTPO (200 ng/mL) for the time indicated. The lysates were subjected to SDS-PAGE and the changes in tyrosine phosphorylation were analyzed by probing with anti-phosphotyrosine MoAb.