Fig. 2.
Kinetics of delivery of antigenic peptides derived from exogenous antigens into class I presentation pathway of DC. bmDC were cultured in the presence of 2 mg/mL ovalbumin and 20 ng/mL GM-CSF for different time periods. Lysis of bmDC pulsed with OVA protein by the OVA-specific CTL clone B3 was assessed in a standard 51Cr-release assay. DC pulsed with 1 μmol/L OVA peptide (DC + OVA peptide) or untreated DC (DC) were included as a control.