The presentation of soluble antigens on MHC class I molecules by DC is altered by proinflammatory mediators. bmDC grown in media containing 20 ng/mL GM-CSF were cultured in the presence or absence of TNF-α (50 ng/mL), IL-12 (50 ng/mL), IL-7 (50 ng/mL), IL-6 (100 ng/mL), IL-4 (20 ng/mL), IFN-γ (100 U/mL), LPS (10 μg/mL), or Flt3L (100 ng/mL). After 24 hours of incubation, 2 mg/mL of soluble ovalbumin was added and 16 hours later the presentation of ovalbumin by bmDC to the OVA-specific B3 clone was analyzed in a standard ⁵¹Cr-release assay. DC pulsed with 1 μmol/L OVA peptide (+OVA peptide) or untreated DC (DC) were included as a control. CTL were added at an E:T ratio of 10:1. The assay was conducted in quadriplicates and error bars show the means and standard deviation.