Identification of the EF-1α gene as an upregulated gene by wild-type p53 in the erythroleukemic cell line 1-2-3, which expresses only the temperature-sensitive p53 (Val-135).28 We identified bands with higher intensity at 32°C than that at 37°C by the DD method using a Delta RNA Fingerprinting Kit (Clontech). One band (D9) was confirmed by Northern blotting analysis (A). (B) Sequence comparison of the D9 clone with the mouse EF-1α gene (GenBank/EMBL m17878). (C) Time course of the expression of the EF-1α gene in 1-2-3 cells and 2-2 cells after the temperature-shift. (D) Comparison of the patterns of expression of several genes that have been suggested to be upregulated by wild-type p53. (E) Western blotting analysis of EF-1α protein. (F ) The pattern of induction of EF-1α protein in 1-2-3 cells after the temperature-shift.