Fig. 7.
![Fig. 7. Conditioning regimes prime macrophages to secrete TNFα after LPS stimulation. Peritoneal macrophages were pooled from a minimum of 4 B6D2F1 animals in each group ([▪] naive; [□] 900cGy TBI; [▧] 1,300 cGy TBI) within 4 hours of the completion of TBI and stimulated with LPS (0.1 μg/mL). TNFα was determined by ELISA in the culture supernatants taken 4 hours after stimulation. Data represent the mean ± standard error of quadruplicate wells standardized for 105 macrophages by FACS analysis using F4/80 positivity. Data represent one of three similar experiments. *P < .03 v naive and 1,300 cGy TBI groups.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/90/8/10.1182_blood.v90.8.3204/4/bl_0004f7.jpeg?Expires=1735550420&Signature=My5KwYBe-m5dX1uG1x1H9viN5lRmKInwvqxOyfvr6ABuqcapBy34c3epZEqdCHwhI7eouHXRIOMZFo~vVU2FM4Ls~REq~3x-VatMqcu2v89IdV6To6EugIZvyD4ANM4xldrRf~ioIUPaiMtxCbIIphh4FjWca73zrECuKOgIF7D79pn6USVDMgBLHrmO1eFRtyl6b0BcusBuWxVGfP0LicEoyk8TBdlAzA3t3-x7eK4f2ODq8I53K3gqLFKDUltLCZZJ-XefpoDxI9Yi2lgf2qL~NFOgE~TQfq3GPnSDB8u3xFqcsgJVobRddzZxqE658hIMDwD0O0qwMEuSWiGabQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Conditioning regimes prime macrophages to secrete TNFα after LPS stimulation. Peritoneal macrophages were pooled from a minimum of 4 B6D2F1 animals in each group ([▪] naive; [□] 900cGy TBI; [▧] 1,300 cGy TBI) within 4 hours of the completion of TBI and stimulated with LPS (0.1 μg/mL). TNFα was determined by ELISA in the culture supernatants taken 4 hours after stimulation. Data represent the mean ± standard error of quadruplicate wells standardized for 105 macrophages by FACS analysis using F4/80 positivity. Data represent one of three similar experiments. *P < .03 v naive and 1,300 cGy TBI groups.
