Separation of early CD34⁺ subsets from mobilized peripheral blood. CD34⁺ cell-enriched suspensions were labeled with FITC-CD34, PE-CD38, PE-CD33, and PerCP-HLA-DR and PI. Viable (PI^low) cells were sorted with an ELITE ESP flow cytometer using predetermined forward and orthogonal light scatter parameters. (A) Gate for CD34⁺ CD38^dim CD33^dim cells. (B) Gated cells from (A) displayed for sorting of HLA-DR^dim cells in the lower gate (25% of events shown) and HLA-DR⁺ cells in the upper gate (61% of events shown).