Fig. 7.
Effect of FL on the number of primitive precursors after cultivation of HLA-DR+ cells and HLA-DRdim cells. Mobilized peripheral blood cells were sorted into HLA-DR+ and HLA-DRdim fractions and cultured in [IL-3 + IL-6 + KL] ± FL. After 6 days, cultures were analyzed for the indicated phenotypes. The figure shows the absolute yield of CD34+ cells and subsets. FL supported a greater yield of CD34+ cells, CD34+ CD33dim cells, and CD34+ HLA-DRdim cells in HLA-DR+ cultures than in the HLA-DRdim cultures. Data are representative of 3 independent trials.