Effects of IFN-γ and IL-10 on membrane expression of TNF-RII (CD120b) in control and LPS-stimulated monocytes. (A) Normal human monocytes (1 × 106 cells/mL) were incubated in medium alone or in medium containing IFN-γ (10 ng/mL), IL-10 (10 ng/mL), or both for 6 hours. Cells were then labeled with PE-conjugated mouse antihuman TNF-RII (CD120b) mAb and analyzed by FACS. (B) Duplicate cultures of normal human monocytes were pretreated for 30 minutes with medium alone or medium containing IFN-γ (10 ng/mL), IL-10 (10 ng/mL), or both. LPS (10 ng/mL) was then added to one of each pair of these cultures and incubated for an additional 6 hours. At the end of the incubation period, cells were labelled with PE-conjugated anti–TNF-RII mAb and analyzed by FACS. Values represent the mean fluorescence intensities of 1 × 104 cells.
