Fig. 1.
![Fig. 1. Temperature gradient gel of TCRγ PCR products. Lanes 1 to 5, polyclonal PCR products of PCR 1 using primers VG1, VG2, VG9, and JG12-a (1 to 3) and PCR 2 using primers VG1, VG2, VG9, and JGP12-a (4 and 5) appearing as broad smears in the middle range of the gel (a). Lanes 6 to 9, clonal controls (cell line JM [Vγ8], cell line PEER [Vγ9], T-ALL patient Ra [Vγ10], and cell line JM [Vγ11]) appearing as sharp bands below the middle range of the gel (b). Lanes 10 to 17, clonal PCR products of PCR 1 (11 to 13) and PCR 2 (14 and 15). Lanes 16 and 17, biallelic PCR products of PCR 1. Lane 18, HincII digest of phi X174. S, skin sample; B, blood sample; C, cell line; M, marker.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/90/4/10.1182_blood.v90.4.1636/3/bl_0051f1.jpeg?Expires=1735541626&Signature=ysUVVA94u8HniSyY9O6n686C77mpbJkrvhXq16idI-UhasAkovNKjLD0nMOonWTwq-kFEbU-sVndVx95UEgdaTVUMxGfofHxhdzPpVbogSofW~5HvkeKgS~1G2UP~3Y-hRRwDd3cb0UIIvWYDS-W93zkxV6une4Bas5uzOwvu86s0fWxcyHlkpE2iAaJrRNeL2NquSDnkd2WAN0jvEbkxpPMgJK8LIZ-v~yXAkNXw6-FbtnmdDEB0r9s5AD23U4vYsTwtuZtaQUzPz4ZFgZbjPYyi~2HxOKrVM9pe6bjl76CreCYbDGwRPL5LuESQzwZeL0ucWZ9HWGc5swPcCwHzg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Temperature gradient gel of TCRγ PCR products. Lanes 1 to 5, polyclonal PCR products of PCR 1 using primers VG1, VG2, VG9, and JG12-a (1 to 3) and PCR 2 using primers VG1, VG2, VG9, and JGP12-a (4 and 5) appearing as broad smears in the middle range of the gel (a). Lanes 6 to 9, clonal controls (cell line JM [Vγ8], cell line PEER [Vγ9], T-ALL patient Ra [Vγ10], and cell line JM [Vγ11]) appearing as sharp bands below the middle range of the gel (b). Lanes 10 to 17, clonal PCR products of PCR 1 (11 to 13) and PCR 2 (14 and 15). Lanes 16 and 17, biallelic PCR products of PCR 1. Lane 18, HincII digest of phi X174. S, skin sample; B, blood sample; C, cell line; M, marker.
