The effect of 7-day serum-free liquid cultures containing IL-3, SCF, IL-6, and αTGFβ1 with or without stroma-conditioned media (SCM) on (A) the expansion of different stem cell subsets (CFC and CAFC weeks 2 to 6) and (B) the ability of stem cells to produce NA CFC in parallel flask long-term cultures (LTC) for 6 weeks. At week 6 the stroma-adherent (SA) CFC content was also determined. Comparison between no SCM and with SCM: *, P < .05. (□), No SCM (n = 7 to 9); (▪), FBMD-1 SCM (n = 7 to 9); (▨), L87/4 SCM (n = 3 to 5); (), L88/5 SCM (n = 3 to 5).