The effect of FBMD-1 SCM, FBMD-1 stroma-contact, and FBMD-1 stroma-noncontact on (A) the expansion of different stem cell subsets (CFC and CAFC week 2 to 6) and (B) the ability of stem cells to produce NA CFC in parallel flask LTC for 6 weeks in 7-day serum-free liquid cultures containing IL-3, SCF, IL-6, and αTGFβ1. At week 6 the stroma-adherent (SA) CFC content also was determined. Comparison between FBMD-1 SCM and FBMD-1 stroma-contact or stroma-noncontact: °, P < .05. Comparison between FBMD-1 stroma-noncontact and FBMD-1 stroma-contact: ^▿, P < .05. (▪), SCM (n = 5 to 6); (□), stroma-contact (n = 5 to 6); (□), stroma-noncontact (n = 3).