Fig. 4.
![Fig. 4. In vitro IFN-γ production from lymph node cells isolated during the period of rmIL-12 treatment. Mice received a single injection of either vehicle (A) or rmIL-12 (0.5 μg) (B) 7 days before daily rmIL-12 administration (days 0 to 5). Lymph nodes (popliteal, axillary, and brachial) were harvested during the period of daily rmIL-12 administration for in vitro cytokine assays. The cells were stimulated with either rmIL-12 (0.1 [] or 1 ng/mL [▪]) or Con A (0.5 μg/mL, ▨) as described in the Materials and Methods section. IFN-γ secretion was measured by a commercially available ELISA (Endogen). (▨), Control.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/90/7/10.1182_blood.v90.7.2541/3/bl_0034f4.jpeg?Expires=1735562697&Signature=CARmBPTa07hicm9vhJvlJWAiaD1O46YcTGvajnjBRbGhWNuCINOfjMj-4b3V01vQJuuiwXM-Bqo7GIH1yuEiGlolc2rfg9ihKX5VUPlo5sH91iI-WOvvcFLbwIkMwFb7yGlQxaQcH4IsUsxqbXkFc-XzG82nxLRJWtVGn9sWoycA2-Jcx~iiN4LiUxTvThE967D4dcE0~m5QiXqvW8MJo5jP0Oy~exg0Dr~Qy9U0VO736-lBOhtfQWVCPYOooNDrzoQxkxaE~Y6QWi4yfjdTQm0FRICulU9Wk40uCKWZOai2NrWbc0ZB6CgfhMzBvwWGQorz6ikla9N-VPzPg-tUzw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
In vitro IFN-γ production from lymph node cells isolated during the period of rmIL-12 treatment. Mice received a single injection of either vehicle (A) or rmIL-12 (0.5 μg) (B) 7 days before daily rmIL-12 administration (days 0 to 5). Lymph nodes (popliteal, axillary, and brachial) were harvested during the period of daily rmIL-12 administration for in vitro cytokine assays. The cells were stimulated with either rmIL-12 (0.1 [] or 1 ng/mL [▪]) or Con A (0.5 μg/mL, ▨) as described in the Materials and Methods section. IFN-γ secretion was measured by a commercially available ELISA (Endogen). (▨), Control.
