Fig. 7.
Fig. 7. Protein expression of the βIT chain. (A) Anti-β immunoprecipitation and Western blotting of COS-7 cells transiently transfected with either βC or βIT. (B) Western blots of cell lysates from COS-7 cells transfected with βC or βIT and probed with either anti-β antibody, anti-βIT antibody, or anti-βIT + specific or unrelated peptide. (C) Western blots of anti-β or anti-βIT immunoprecipitates from hematopoietic cell lines (TF-1 and CTLL) or primary CD34+cells cultured for 8 days in stem cell factor, IL-3, and IL-6 and probed with anti-β antibody.

Protein expression of the βIT chain. (A) Anti-β immunoprecipitation and Western blotting of COS-7 cells transiently transfected with either βC or βIT. (B) Western blots of cell lysates from COS-7 cells transfected with βC or βIT and probed with either anti-β antibody, anti-βIT antibody, or anti-βIT + specific or unrelated peptide. (C) Western blots of anti-β or anti-βIT immunoprecipitates from hematopoietic cell lines (TF-1 and CTLL) or primary CD34+cells cultured for 8 days in stem cell factor, IL-3, and IL-6 and probed with anti-β antibody.

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