Effect of C2-ceramide, calcium ionophore, and forskolin on viability of the CD77⁺ Daudi BL cells. Cells were cultured for 24 hours with various concentrations of C2-cer (from 5 to 30 μmol/L), calcium ionophore A23187 (from 0.1 to 5 μmol/L), or forskolin (0.5 and 1 mmol/L) and also with combinations of these molecules: C2-cer (5 μmol/L) + forskolin (1 mmol/L), A23187 (0.1 μmol/L) + forskolin (1 mmol/L), and C2-cer (5 μmol/L) + A23187 (0.1 μmol/L). The percentage of cell viability was obtained by propidium iodide (PI) staining and FACS analysis.