hsp90, PP1Cα, and PP1M are released from the hsc70 phosphoprotein complex during platelet adhesion to collagen. Anti-hsc70 immunoprecipitates from lysates of resting (Cont) and adherent (Adh) platelets were separated by SDS-PAGE minigels, and Western blots were performed with the appropriate antibodies as described in the Materials and Methods. (A) Equal amounts of hsc70 were immunoprecipitated from resting and adherent platelets. (B) hsp90 dissociates from hsc70, (C) PP1Cα dissociates from hsc70, and (D) PP1M dissociates from hsc70 when platelets adhere to collagen.