Translocation of hsp90 from the cytoplasm to the cytoskeleton and membrane skeleton during platelet adhesion to collagen. Resting and adherent platelets were solubilized in lysis buffer and cytoskeletal (csk), membrane-skeleton (msk), and cytosol (cyt) fractions were prepared by using differential centrifugation.30 The fractions were subjected to 8% SDS-PAGE and transferred to nitrocellulose membranes. The presence of hsp90 in each fraction was examined by Western blot analysis using the hsp90 antibody AC88. The location of hsp90 is indicated by the arrow. One of two similar experiments is shown.