Surface phenotype of prototypic HVS-Normal (HVS-N8) and HVS-WAS (HVS-WAS1) immortalized T-cell lines. Flow cytometric analysis shows that HVS-Normal and HVS-WAS T-cell lines have similar surface expression of CD2, CD3, CD4, CD25, CD28, and CD43 isoforms (MoAb L10 recognizes tetrasaccharide and hexasaccharide forms of CD43, whereas MoAb T305 reacts exclusively with the hexasaccharide isoform of the molecule), CD45, and HLA-DR. The two cell lines shown in the figure and all other cell lines obtained are CD56⁻.