Fig. 3.
![Fig. 3. Proliferative responses of HVS-Normal (HVS-N8 [•]) and HVS-WAS (HVS-WAS1 [▵] and HVS-WAS7 [▴]) immortalized T-cell lines after stimulation with immobilized OKT3 MoAb and allospecific Raji cells. Overnight-starved T cells were plated (105 cells/well) in 96-well plates that had been precoated with 10, 25, 50, or 100 ng/well of OKT3 MoAb (left panel) or cocultured in the presence of 25, 50, or 100 × 103 mitomycin-C treated allospecific Raji cells (right panel). After 48 hours of culture, cells were labeled with [3H]-thymidine for an additional 16 hours. Thymidine incorporation was determined by liquid scintillation counting.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/90/8/10.1182_blood.v90.8.3089/4/bl_0026f3.jpeg?Expires=1735862080&Signature=O5A9N9d1b~C5TtqwThvpEhITqBsoDWpFFN2G33CWAOPTsFxjQ8PzTet2pSmOtc5MSd7AZn5jXfEemeokhG5IPS6n9FFwnCQhfZnKWoXC9Sp~NrjmziC0Q90pW9yKxVbrLQmfjwdtZtZB0nDb~MOe3jUtBC4~SxfVsDgYnEKLeYBmM0GAC75raar8ysxTADXzFSoKLdr-IutxQdCCTnT1JXHUbHnfkETvKh2N4YBngbym4SYRP-OpTkpkx0Y2Qmwm-9y-pY5bOqTV0-qS431qTCWjkMZPQ-oOxfA1Xa~GM6nliRWcdfJb-fBtjbwp6ErQviKRpi~6kLa0KgjCmyrkxw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Proliferative responses of HVS-Normal (HVS-N8 [•]) and HVS-WAS (HVS-WAS1 [▵] and HVS-WAS7 [▴]) immortalized T-cell lines after stimulation with immobilized OKT3 MoAb and allospecific Raji cells. Overnight-starved T cells were plated (105 cells/well) in 96-well plates that had been precoated with 10, 25, 50, or 100 ng/well of OKT3 MoAb (left panel) or cocultured in the presence of 25, 50, or 100 × 103 mitomycin-C treated allospecific Raji cells (right panel). After 48 hours of culture, cells were labeled with [3H]-thymidine for an additional 16 hours. Thymidine incorporation was determined by liquid scintillation counting.
