Anti-WASP antisera. (A) WASP-derived peptides used in the production of rabbit anti-WASP antibody (Ab). (B) Assessment of Ab953, Ab503, and Ab1468 using Western blot analysis of normal B-LCL lysate and preimmunization and postimmunization antisera. (C) Only protein translated from WASP gene mRNA, not from luciferase gene mRNA, was recognized by Ab503 as a single band of 62 kD, demonstrating that the antibody was specific for WASP.