Fig. 7.
Zymography analysis of coculture media. Stromal cells were seeded in 96-well plates at a concentration of 1.5 × 104 cells/100 μL/well. Myeloma cells (1.25 × 105 cells/well) were added to stromal cells, and supernatants were harvested after 48 hours of coculture. Lane 1, monoculture of BMSC; lane 2, coculture of BMSC and myeloma cells; lane 3, monoculture of myeloma cells. Three different experiments are shown.