American Society of Hematology

Fig. 4.

$Fig. 4. Cell fractionation studies of rabbit reticulocytes at various calcium concentrations. Cell preparation, hemolysis, sample work-up, and immunoblotting are described in the Materials and Methods section. (A) Cytosol fraction; (B) membrane pellet; (C) supernatant of washing (washed at 10−3 mol/L calcium); (D) washed membrane pellet (washed at 10−3 mol/L calcium); (E) cytosol fraction; (F) membrane pellet; (G) supernatant of washing (washed at 10−6 mol/L calcium); (H) washed membrane pellet (washed at 10−6 mol/L calcium); (I) cytosol; (K) membrane pellet; (L) supernatant of washing (washed at 5 × 10−8 mol/L calcium); (M) washed membrane pellet (washed at 5 × 10−8 mol/L calcium).$

Cell fractionation studies of rabbit reticulocytes at various calcium concentrations. Cell preparation, hemolysis, sample work-up, and immunoblotting are described in the Materials and Methods section. (A) Cytosol fraction; (B) membrane pellet; (C) supernatant of washing (washed at 10⁻³ mol/L calcium); (D) washed membrane pellet (washed at 10⁻³ mol/L calcium); (E) cytosol fraction; (F) membrane pellet; (G) supernatant of washing (washed at 10⁻⁶ mol/L calcium); (H) washed membrane pellet (washed at 10⁻⁶ mol/L calcium); (I) cytosol; (K) membrane pellet; (L) supernatant of washing (washed at 5 × 10⁻⁸ mol/L calcium); (M) washed membrane pellet (washed at 5 × 10⁻⁸ mol/L calcium).

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