PCR detection of BCL2/JH rearrangement. Two representative results from unprocessed and processed BM are shown. Left panel: Data from patient 19 (disappearance of the BCL2/JH translocation in the CD34⁺ cell fraction). Right panel: Data from patient 14 (persistance of the BCL2/JH translocation in the CD34⁺ cell fraction). Control⁺, RL cells; Control⁻, normal PB lymphocytes; BC, DNA extracted from buffy coat (unprocessed BM); CD34⁻, DNA extracted from unadsorbed CD34⁻ cell fraction; CD34⁺, DNA extracted from adsorbed CD34⁺ cell fraction; MW, molecular weight markers. Arrows show the specific BCL2/JH⁺ band.