Fig. 2.
Kinetics of PU.1 expression in G-CSF–treated 32Dcl3 cells. Total RNA (A) was isolated at the indicated times, and 5 μg of each sample was electrophoresed, blotted, and hybridized to a 32P-labeled PU.1 cDNA probe. Total lysate (30 μg) was used for immunoblotting (B) with the anti-PU.1 antibody.
