Effect of the addition of rmSCF on the expression of MMCP-5, MMCP-6, or MC-CPA gene in +/+ and mi/mi CMCs. (A) Northern blotting. Total RNA was obtained from +/+ or mi/mi CMCs cultured in the presence of 10% PWM-SCM and 10 ng/mL SCF for 0, 1, 2, or 3 weeks. The blot was hybridized with ³²P-labeled cDNA probes for MMCP-5, MMCP-6, and MC-CPA. (B) RT-PCR analysis for the expression of MMCP-5, MMCP-6, or MC-CPA gene. The same RNAs were used as those used in Northern blotting. PCR products were electrophoresed in 1.0% agarose gel containing ethidium bromide.