Fig. 7.
![Fig. 7. Effect of ATP and adenosine on [Ca2+]i. HUVECs were grown on gelatin-coated glass coverslips, loaded with fura-2, and immersed in a quartz cuvette for radiometric fluorescent emission measurements. Each tracing shown is representative of at least 3 similar experiments. (A) Stimulation with 100 μmol/L ATP was followed by 0.5 U/mL thrombin (Thr). The [Ca2+]i response to thrombin looks virtually identical when tested alone, and is not modified by prior stimulation with ATP (not shown). (B and C) The response to 2-MeS-ATP was similar to the ATP response (B), and was not modified by preincubation with IBMX and adenosine (Ado), both tested at 100 μmol/L (C).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/91/1/10.1182_blood.v91.1.118/3/blod4011507.jpeg?Expires=1735545869&Signature=mG6Y7YLKEu-s-SlgHfRnsjpXpxqUoE7JJPJVC3la-YRfTOpsf9rsbHEO5VOprEkriwPRNYD1sZzY7BrHnNXfkgGd2q240j8ad6QirLy5MdgD1nhbcmEzxpiVkUcqF3k4IfCnhHqmq6Nw9ElbryH75wrzH2rskTJaAEw4VrZRyl-gookq68oUl3FOOozmMiBzUd5~PZVMZlUlvmz5vfaVk9xDK92vwgFUdXQzF5hp~AFtT6pmSNh5uoqMnoIJyJQvmH6pdYcwkvQS9Y9UCFkqhcbBcHNAGWqiHYFtgUF3yBWf4RD1YW2yUbPBXAUwMUQzjZmtTeQsrbrcmddTXWZ96w__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effect of ATP and adenosine on [Ca2+]i. HUVECs were grown on gelatin-coated glass coverslips, loaded with fura-2, and immersed in a quartz cuvette for radiometric fluorescent emission measurements. Each tracing shown is representative of at least 3 similar experiments. (A) Stimulation with 100 μmol/L ATP was followed by 0.5 U/mL thrombin (Thr). The [Ca2+]i response to thrombin looks virtually identical when tested alone, and is not modified by prior stimulation with ATP (not shown). (B and C) The response to 2-MeS-ATP was similar to the ATP response (B), and was not modified by preincubation with IBMX and adenosine (Ado), both tested at 100 μmol/L (C).
