(A) Phenotypic analysis of thymocytes from wt control and hSCF²²⁰ transgenic mice. Total thymocytes from an hSCF²²⁰ homozygous transgenic and wt control littermates were stained with FITC-conjugated CD4 MoAb and PE-conjugated CD8 MoAb, and analyzed by flow cytometry (upper panel) or FITC-conjugated CD8 MoAb and PE-conjugated CD3 MoAb (lower panel), and analyzed by flow cytometry. Data are from a representative group of mice. Numbers in quadrants indicate the percentage of the total population from a representative group of mice. Three wt control and three hSCF²²⁰ mice were examined. (B) Phenotypic analysis of T-cell subsets in spleens of wt control and hSCF²²⁰ transgenic mice. Spleen cells from an hSCF²²⁰ homozygous transgenic mouse and wt control littermate were stained with FITC-conjugated CD4 MoAb (top and middle panels) and PE-conjugated CD8 and CD3 MoAb (top and middle panel) or stained with FITC-conjugated CD8 MoAb and PE-conjugated CD3 MoAb (lower panel), and analyzed by flow cytometry. Numbers in quadrants indicate the percentage of the total population. Three wt control and three hSCF²²⁰ mice were examined.