Kinetics of amplification of ICs. (A) Amounts of amplification products (cpm) of the IC MACM (5 × 10⁴copies per PCR reaction) were measured after the first round (▪) and second round of nested PCR (○), and plotted against the number of PCR cycles. Results are means of two experiments in triplicate. (B) Products generated after PCR amplification of 5 × 10⁴copies of plasmid IC (MACM) (•) were compared with those generated after amplification of genomic DNA (5 × 10⁴ RL cells) (□).