Fig. 3.
Example for analysis of long-term chimerism. Spleen cells of surviving animals from the experiment described in Figs 1 and 2 were collected 100 days after transfer of 1 × 109 DBA PBPC/kg BW in Balb/c mice. The mononuclear cells were prepared by density gradient centrifugation and stained with MoAb anti-Lyt 1.1. Lyt 1.1 is an isoform of murine CD5 found in DBA but not in Balb/c mice. Untreated Balb/c and DBA mice served as negative and positive controls, respectively. The transplant recipients showed the same percentage of Lyt 1.1+ splenocytes as DBA mice, demonstrating long-term chimerism of T cells after transplantation.
