American Society of Hematology

Fig. 2.

$Fig. 2. Histone acetylation in K562 cells after treatment with butyrate, histone deacetylase inhibitors, or hydroxyurea. Cells were treated for 24 hours with the arginine-butyrate (1 mmol/L), trapoxin (2.5 nmol/L), HC toxin (10 nmol/L), trichostatin A (0.5 μmol/L), or hydroxurea (1 mmol/L), after which histones were isolated (see Materials and Methods) and fractionated on acid/urea gels. A gel stained with Coomassie blue is shown. The identities of the H4 bands were verified by protein microsequencing. The lowest band in each lane is unacetylated H4, and the numbers to the left indicate the number of acetylated lysines in each of the higher bands.$

Histone acetylation in K562 cells after treatment with butyrate, histone deacetylase inhibitors, or hydroxyurea. Cells were treated for 24 hours with the arginine-butyrate (1 mmol/L), trapoxin (2.5 nmol/L), HC toxin (10 nmol/L), trichostatin A (0.5 μmol/L), or hydroxurea (1 mmol/L), after which histones were isolated (see Materials and Methods) and fractionated on acid/urea gels. A gel stained with Coomassie blue is shown. The identities of the H4 bands were verified by protein microsequencing. The lowest band in each lane is unacetylated H4, and the numbers to the left indicate the number of acetylated lysines in each of the higher bands.

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