Fig. 3.
![Fig. 3. Exposure of P-selectin on the surface of single platelets during adhesion to fibrinogen or collagen matrices. Fura-2–loaded platelets were allowed to adhere to immobilized fibrinogen (A) or collagen (B) in the presence of 2 mmol/L CaCl2 and FITC-labeled AK-6 antibody against P-selectin. Arrows indicate timepoint of addition of 2 nmol/L thrombin or timepoint of attachment to collagen. Fluorescence images were taken during 5 to 10 minutes, and traces represent changes in FITC fluorescence of individual platelets. These platelets were high in [Ca2+]i at the end of the FITC fluorescence recordings. Traces are representative of >35 platelets out of three independent experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/90/7/10.1182_blood.v90.7.2615/3/bl_0025f3.jpeg?Expires=1735828787&Signature=TrwSlgQb1NcXtB~BXdWXqYUBlDqc1Pq177NoG3BvN~vV7NXuNqetjDk~Muz8ApBIID~zhw8mw-WKWAOr4pKzKsaXLbuzYL~toYCi4EFqhz0yhl10auYDE9qn9VhGL~FniyI7ZcAOYSYgHo5nF96xNC1-mZyB9Eu5O1BImYdH6IUQSIbZV0PPChXpKB9E4TwHtlvOq57gVjcmJpBTaS~e-Y-UcyIMcsiBqATsKXD4MRCLUFdj2r1f9dYHlbeN8XGgvBaAS~GwHBYqpG~YxPYYmc9v8wWXaXtlLYqffJ4ulM3ULTfzGkWNMpsH8XB1lkMc2siENuV39WWKd4~6YXQTYQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Exposure of P-selectin on the surface of single platelets during adhesion to fibrinogen or collagen matrices. Fura-2–loaded platelets were allowed to adhere to immobilized fibrinogen (A) or collagen (B) in the presence of 2 mmol/L CaCl2 and FITC-labeled AK-6 antibody against P-selectin. Arrows indicate timepoint of addition of 2 nmol/L thrombin or timepoint of attachment to collagen. Fluorescence images were taken during 5 to 10 minutes, and traces represent changes in FITC fluorescence of individual platelets. These platelets were high in [Ca2+]i at the end of the FITC fluorescence recordings. Traces are representative of >35 platelets out of three independent experiments.
