Fig. 4.
Fig. 4. DNA binding activities of NFAT and AP-1, but not NFκB and CD28RC, are enhanced by IL-7 in activated human T lymphocytes. Nuclear extracts were prepared from unstimulated T cells and T cells stimulated for 2 hours with PHA or PHA plus anti-CD28 (aCD28) in the presence or absence of IL-7. EMSAs were performed with probes comprising (A) the distal NFAT site, (B) the proximal AP-1 site, (C) the NFκB site, and (D) the CD28 response element in the human IL-2 promoter. DNA binding activities were quantified using a PhosphorImaging system. The lower graphs show the mean ± SEM found for the DNA binding activities of NFAT, AP-1, NFκB, and CD28RE in four independent experiments. The results are expressed as relative DNA binding compared to the PHA-induced DNA binding, which was set at 1.

DNA binding activities of NFAT and AP-1, but not NFκB and CD28RC, are enhanced by IL-7 in activated human T lymphocytes. Nuclear extracts were prepared from unstimulated T cells and T cells stimulated for 2 hours with PHA or PHA plus anti-CD28 (aCD28) in the presence or absence of IL-7. EMSAs were performed with probes comprising (A) the distal NFAT site, (B) the proximal AP-1 site, (C) the NFκB site, and (D) the CD28 response element in the human IL-2 promoter. DNA binding activities were quantified using a PhosphorImaging system. The lower graphs show the mean ± SEM found for the DNA binding activities of NFAT, AP-1, NFκB, and CD28RE in four independent experiments. The results are expressed as relative DNA binding compared to the PHA-induced DNA binding, which was set at 1.

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