The enhanced DNA binding activity of AP-1 induced by IL-7 in activated human T lymphocytes is CsA-resistant, while the NFAT DNA binding activity is CsA-sensitive. (A) Nuclear extracts were prepared from T cells stimulated for 2 hours with PHA plus anti-CD28 (aCD28) in the presence or absence of IL-7. CsA was added 30 minutes before stimulation. EMSAs were performed as described in Fig 3. (B) At the same time, cells were stimulated for 24 hours for IL-2–secretion measurements. The mean for the IL-2 secretion found in four independent experiments is shown.