American Society of Hematology

Fig. 1.

$Fig. 1. Expression of GM-CSF receptors in LNCaP cells. (A) RT-PCR analysis. Total RNA was isolated from LNCaP (lane 1) or HL-60 (lane 2) cells and subjected to RT-PCR using radiolabeled primers specific for the α (left panel) or the β (right panel) subunits of the GM-CSF receptor. PCR products were size-fractionated on 5% acrylamide gels and autoradiographed. Shown are the PCR products corresponding to the α (370 bp) and the β (570 bp) subunits of the GM-CSF receptor obtained in the presence (+) or in the absence (-) of reverse transcriptase (RT). (B) Binding analysis. Cells were incubated with radiolabeled GM-CSF at concentrations that ranged from 5 pmol/L to 10 nmol/L. GM-CSF binding was dose dependent and saturable approximately at 10 nmol/L. (C) Scatchard analysis of data from (B) showing the presence of two classes of binding sites in the LNCaP cells. (D through G) Immunostaining with antihuman GM-CSF receptor antibodies. Cells were cultured, fixed, and incubated with anti-α (D and F) or anti-β subunit (E and G) antibodies in the absence (D and E) or the presence (F and G) of the peptides used to elicit them (original magnification × 160).$

Expression of GM-CSF receptors in LNCaP cells. (A) RT-PCR analysis. Total RNA was isolated from LNCaP (lane 1) or HL-60 (lane 2) cells and subjected to RT-PCR using radiolabeled primers specific for the α (left panel) or the β (right panel) subunits of the GM-CSF receptor. PCR products were size-fractionated on 5% acrylamide gels and autoradiographed. Shown are the PCR products corresponding to the α (370 bp) and the β (570 bp) subunits of the GM-CSF receptor obtained in the presence (+) or in the absence (-) of reverse transcriptase (RT). (B) Binding analysis. Cells were incubated with radiolabeled GM-CSF at concentrations that ranged from 5 pmol/L to 10 nmol/L. GM-CSF binding was dose dependent and saturable approximately at 10 nmol/L. (C) Scatchard analysis of data from (B) showing the presence of two classes of binding sites in the LNCaP cells. (D through G) Immunostaining with antihuman GM-CSF receptor antibodies. Cells were cultured, fixed, and incubated with anti-α (D and F) or anti-β subunit (E and G) antibodies in the absence (D and E) or the presence (F and G) of the peptides used to elicit them (original magnification × 160).

This Feature Is Available To Subscribers Only