Establishment of cell lines that ectopically express HOX-B8. (A) Northern blot analysis of HOX-B8 expression in M1, HL-60, and 32Dcl3 derivatives, established following transfection with either pMLV or pMLV-HOX-B8, and WEHI-3B. 10 μg of total RNA was analyzed by Northern blots as described in Materials and Methods. (B) Southern blot analysis of genomic DNA from parental 32Dcl3, 32Dneo, and 32DHOX-B8 clones. Genomic DNA (l0 μg) was digested with EcoRI, resolved on a 1% agarose gel, transferred to Gene Screen Plus (NEN), and hybridized to a murine HOX-B8 cDNA probe.