Fig. 1.
Successful intraocular gene transfer using replication-deficient adenovirus vector in a nude mouse model. (A) β-Galactosidase reporter gene expression in intraocular human uveal melanomas treated in oculi with AdCMV-βGal vector. (B) PAI-1 antigen expression in human uveal melanoma treated in oculi with AdCMV-PAI-1 vector. A murine IgG1 antihuman PAI-1 MoAb was used to detect human PAI-1 expression in paraffin-embedded eyes using an immunoperoxidase procedure described elsewhere. (C) Absence of immunohistochemical staining in OCM-1 melanomas treated with AdCMV-PAI-1 and exposed to peroxidase-conjugated secondary antibody and substrate. c, cornea; uv, uveal melanoma. Bar = 80 μm.
