American Society of Hematology

Fig. 1.

$Fig. 1. Association of CD44 with low-density plasma membrane fractions in human T lymphocytes. One hundred million human PBMC (left panel) or PHA blasts (right panel) were extracted in TKM buffer containing 0.5% TX-100, and the lysates were subjected to equilibrium gradient centrifugation as described in the Materials and Methods. Eleven 1-mL fractions were collected from the top, and 20 μL of each fraction (2 through 11) was electrophoresed under nonreducing (CD44, CD45, MHC-I, and CD59) or reducing (Lck and Fyn) conditions. Separated proteins were detected by ECL-based Western blot. Fractions 3 through 6 correspond to the 5% to 36% sucrose interface. Data shown are representative of at least three experiments. Identical distribution profiles were obtained when 0.5% Brij-58 was used for extraction. Fraction 1 did not contain any of the cell surface or intracellular proteins tested.$

Association of CD44 with low-density plasma membrane fractions in human T lymphocytes. One hundred million human PBMC (left panel) or PHA blasts (right panel) were extracted in TKM buffer containing 0.5% TX-100, and the lysates were subjected to equilibrium gradient centrifugation as described in the Materials and Methods. Eleven 1-mL fractions were collected from the top, and 20 μL of each fraction (2 through 11) was electrophoresed under nonreducing (CD44, CD45, MHC-I, and CD59) or reducing (Lck and Fyn) conditions. Separated proteins were detected by ECL-based Western blot. Fractions 3 through 6 correspond to the 5% to 36% sucrose interface. Data shown are representative of at least three experiments. Identical distribution profiles were obtained when 0.5% Brij-58 was used for extraction. Fraction 1 did not contain any of the cell surface or intracellular proteins tested.

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