American Society of Hematology

Fig. 2.

$Fig. 2. Distribution of the glycosphingolipid GM1 in equilibrium sedimentation gradients. In (A), 10 μL of the gradient fractions shown in Fig 1 was dot-blotted onto nitrocellulose filters, blocked, and probed with HRP-conjugated cholera toxin followed by ECL detection. The total protein profile of the gradient fractions of TX-100 lysate of PBMC is shown in (B). A similar profile obtained for PHA blasts is not shown.$

Distribution of the glycosphingolipid GM1 in equilibrium sedimentation gradients. In (A), 10 μL of the gradient fractions shown in Fig 1 was dot-blotted onto nitrocellulose filters, blocked, and probed with HRP-conjugated cholera toxin followed by ECL detection. The total protein profile of the gradient fractions of TX-100 lysate of PBMC is shown in (B). A similar profile obtained for PHA blasts is not shown.

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