Fig. 5.
Irradiated WEHI-3 cells mixed with gene-modified fibroblasts expressing CD40L have no effect on the growth of preexisting tumor. Four groups of mice (seven per group) were challenged with 105 WEHI cells on day 1. On days 4 and 14 mice received SQ injection of 105 WEHI cells mixed with 2 × 105 transduced CL7.1 fibroblasts expressing either the CD40L or IL-2 gene, or neo (control). Before injection, the cell mixture was irradiated to 1,000 cGy. The fourth group of mice was treated with irradiated WEHI cells and a combination of fibroblasts expressing either CD40L or IL-2. Tumor volumes are shown as the mean mm3 ± SEM.
