Immunoblot of PKC isoforms present in TF-1 cells. Exponentially growing TF-1 cells were lysed and proteins were separated by electrophoresis through 10% sodium dodecyl sulfate (SDS) polyacrylamide gels. Separated proteins were analyzed by immunoblotting with a series of commercially available isoform specific antibodies (Transduction Laboratories). The isoforms were visualized by chemiluminescence and exposure to x-ray film.