Time-course for TNFR55 and TNFR75 expression in the presence and absence of PAF. (A) Flow cytometric analysis of CD2 (negative control), TNFR55, and TNFR75 receptor expression in freshly isolated neutrophils. Histograms represent profiles of cell count against log fluorescence and are representative examples from nine separate experiments. (B and C) Human neutrophils (5 × 106/mL) were incubated in MDM containing 10% autologous serum in the presence or absence of PAF (1 μmol/L) for 5 minutes before incubation for 1 to 6 hours in flexiwell plates at 37°C. At these time points TNFR55 (B) and TNFR75 (C) expression was quantified by flow cytometry as detailed in Materials and Methods. Data represent percent of specific receptor expression values obtained in freshly isolated cells with each point being the mean ± SEM of three separate experiments, each performed in triplicate.
