Fig. 6.
(A) K562 erythroleukemia cells express EPO. Total protein cell lysates (10 μg/lane) from K562 cells, from rhEPO stimulated BFU-E colonies, and from HepG2 cells were analyzed by immunoblotting with anti-EPO antibodies. rhEPO (1 U/lane) is shown on lane 1. (B) K562 cells secrete EPO in response to stimulation with EMP. EMP1 was used (0.1 μmol/L represents optimal stimulatory concentration) for stimulation of K562 and HepG2 cells (5 × 106 cells/mL) in serum-free media for 16 hours. The EPO concentration was measured in supernatant by ELISA29 (EMP1 does not crossreact with anti-EPO antibodies). Error bars represent EPO concentration ± standard deviation.
