Fig. 3.
Ca2+ responses to TR agonists in MEG-01 cells. Indo-1–loaded MEG-01 cells were stimulated with thrombin or SFLLRN either in the absence (solid line) or the presence (dotted line) of 2.0 mmol/L EGTA, and intracellular [Ca2+] was measured using a spectrofluorimeter. (A) 5 nmol/L thrombin, (B) 50 nmol/L thrombin, (C) 4 μmol/L SFLLRN, and (D) 40 μmol/L SFLLRN.