Time course of aPTT-F.IX assays in hemophilia B mice following injection of 1 × 10¹¹ particles of an adenoviral vector expressing human F.IX. Citrated plasma was collected and aPTT-F.IX assays performed at the indicated time points. Each symbol represents an individual animal, with each point an average of duplicate measurements. (A) C57B1/6 hemophilic mice. (B) CD-1 hemophilic mice. Mouse 7 is a normal littermate treated with the same dose of vector. (□), 1; (◊), 2; (○), 3; (▵), 4; (⊞), 5; (⧫), 6; (•), 7.