Number of rolling 32Dcl3 cells on P-selectin-IgG but not on E-selectin-IgG is blocked by anti–PSGL-1 MoAb 2PH1. Flow adhesion assays were performed with 32Dcl3 cells in a planar laminar-flow chamber containing cover slips coated with human IgG (hIgG), P-selectin-IgG (P-Sel-IgG), or E-selectin-IgG (E-Sel-IgG). Before the assay, cells were incubated with fivefold-concentrated hybridoma medium (Co), a fivefold-concentrated hybridoma supernatant of 2PH1 (2PH1), or a fivefold-concentrated hybridoma supernatant of the anti–Mac-1 antibody M1/70 (M1/70).