Inhibitory effect of the anti–PSGL-1 MoAb 2PH1 on leukocyte recruitment in vivo. Mice were intravenously injected with either 50 μg (in 200 μL PBS) of the nonblocking control antibody 9DB3 against VCAM-1 (Co), 50 μg of the anti–P-selectin MoAb RB40.34 (RB.40), or 50 μg MoAb 2PH1 (2PH1), immediately followed by IP administration of thioglycollate. Peritoneal leukocytes were removed at 2 hours (A) or 4 hours (B) after stimulation, and neutrophil counts were determined. For the 4-hour time point, 2PH1 was injected twice at time 0 and at 2 hours (50 μg each). The data represent the mean ± SEM of ≥ 4 mice in each group. The depicted experiment represents 1 of 3 (for other time points) independent experiments with similar results.