Effects of antibodies against PSGL-1, P-selectin, and L-selectin on neutrophil recruitment in vivo. Mice were intravenously injected with 9DB3 (Co), 2PH1 (2PH1), anti–P-selectin RB40.34 (RB.40), a mixture of RB40.34 and 2PH1 (RB.40 + 2PH1), anti–L-selectin MEL14 (MEL14), a mixture of MEL14 and 2PH1 (MEL14 + 2PH1), and a mixture of MEL14 and RB40.34 (MEL14 + RB.40). Peritoneal leukocytes were removed at 4 hours after stimulation, and neutrophil counts were determined. Fifty micrograms of each antibody was injected. 2PH1 was injected twice at time 0 and at 2 hours (50 μg each). Data represent the mean ± SEM of ≥ 4 mice in each group. The depicted experiment represents 1 of 3 (for other time points) independent experiments with similar results.