American Society of Hematology

Fig. 1.

$Fig. 1. (A) Rechromatography of crotalin on Mono-S column. The active fraction was dissolved in 0.02 N ammonium acetate, pH 5.0, and loaded on Mono-S column. Elution was carried out with a gradient from 0 to 0.75 mol/L NaCl as indicated (---) at a flow rate of 0.5 mL/min. The active fraction (*) was collected. (B) Gel filtration chromatography on Superose HR 10/30 column. The active fraction above was applied (5 mg) to this column equilibrated with 0.05 mol/L phosphate buffer (pH 7.2). The column was eluted at a flow rate of 0.25 mL/min with the same buffer. The absorbance profile monitored at 280 nm was shown. The active fraction (*) which was eluted at 70 minutes was named crotalin.$

(A) Rechromatography of crotalin on Mono-S column. The active fraction was dissolved in 0.02 N ammonium acetate, pH 5.0, and loaded on Mono-S column. Elution was carried out with a gradient from 0 to 0.75 mol/L NaCl as indicated (---) at a flow rate of 0.5 mL/min. The active fraction (*) was collected. (B) Gel filtration chromatography on Superose HR 10/30 column. The active fraction above was applied (5 mg) to this column equilibrated with 0.05 mol/L phosphate buffer (pH 7.2). The column was eluted at a flow rate of 0.25 mL/min with the same buffer. The absorbance profile monitored at 280 nm was shown. The active fraction (*) which was eluted at 70 minutes was named crotalin.

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